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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3532P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Epithelial Sodium Channel-β Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin in PBS containing 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Epithelial Sodium Channel-β Antibody is validated for use in ELISA, WB for the detection of Epithelial Sodium Channel-β. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. 83-90kDa bands, membrane preparations are recommended (Masilamani et Al., 1999). ELISA: 0.5-1.0 mg/mL using 1 mg/mL control peptide to coat plate. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 20 amino acid peptide sequence near the N-terminus of rat ENaC beta. The immunogen sequence is 100% conserved in mouse, 94% in rabbit, 81% in human, 76% in ovine and 64% in xenopus. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat Epithelial Sodium Channel beta (ENaC beta). The immunogen shows no significant sequence homology with other ENaC subunits. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P51168 # Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. SIZE: 640 amino acids; 72659 Da SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note=Apical membrane of epithelial cells. PTM: Phosphorylated on serine and threonine residues (By similarity). DISEASE: SwissProt: P51168 # Defects in SCNN1B are a cause of autosomal recessive pseudohypoaldosteronism type 1 (PHA1) [MIM:264350]. PHA1 is a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal recessive form that is severe, and the dominant form which is more milder and due to defects in mineralocorticoid receptor. Autosomal recessive PHA1 is characterized by an often fulminant presentation in the neonatal period with dehydration, hyponatraemia, hyperkalaemia, metabolic acidosis, failure to thrive and weight loss. & Defects in SCNN1B are a cause of Liddle syndrome [MIM:177200]. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel. SIMILARITY: SwissProt: P51168 ## Belongs to the amiloride-sensitive sodium channel family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |