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Millipore This Anti-Thiazide-Sensitive Nacl Cotransporter Antibody Is Validated For Use In Ih, Wb For The Detection Of Thiazide-Sensitive Nacl Cotransporter. - Mill (Additional S&H or Hazmat Fees May Apply)
Affinity Purified immunoglobulin in PBS containing no preservatives.
Quality Level
MQ100
Applications
Application
This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.
Key Applications
Immunohistochemistry
Western Blotting
Application Notes
Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended.
Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Synthetic peptide from the N-terminal of human/rat/mouse NCC.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.
Species Reactivity
Human
Mouse
Rat
Rabbit
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_000339.1
Gene Symbol
SLC12A3
NCCT
TSC
Purification Method
ImmunoAffinity Purified
UniProt Number
P55017
UniProt Summary
FUNCTION: SwissProt: P55017 # Electrically silent transporter system. Mediates sodium and chloride reabsorption. SIZE: 1021 amino acids; 113139 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Predominant in kidney. DISEASE: SwissProt: P55017 # Defects in SLC12A3 are the cause of Gitelman syndrome (GS) [MIM:263800]. GS is an autosomal recessive disease characterized by diverse abnormalities in electrolyte homeostasis including hypokalaemic metabolic alkalosis. GS is a subset of Bartter syndrome. SIMILARITY: SwissProt: P55017 ## Belongs to the SLC12A transporter family.
Molecular Weight
160-190 kDa
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
