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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H, B | ELISA, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3560P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1 |
| Alternate Names |
|
| Background Information | The Na+/K+/Cl- Cotransporter isoform 1 (NKCC1) is involved in the transport of sodium, potassium, and chloride ions across membranes. NKCC1 mediates sodium and chloride reabsorption and plays an important role in cell volume regulation. NKCC1 is also involved in the control of normal cell proliferation, while its overexpression results in apparent cell transformation, in a manner similar to some protooncogenes (Panet, 1999). NKCC1 is inhibited by bumetanide and is also referred to as the bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1. Inhibition of NKCC1 activity by bumetanide decreases the high-[K+]o-mediated uptake of Cl , blocks astrocyte swelling, and leads to cell shrinkage under isotonic conditions in cells such as retinal pigment epithelia, ventricular myocytes, and vascular endothelial cells (Russell, 2000; Su, 2002). NKCC1 could contribute to an overload of intracellular Na+, K+, and Cl- and cell swelling in conditions such as cerebral ischemia (Yan, 2001). NKCC1 is expressed in multiple tissues with the amount of mRNA expression varying as much as thirty-fold. It is highly expressed in neurons and astrocytes through-out the brain and has been implicated in ischemic necrotic cell death. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified rabbit polyclonal in buffer containing PBS with 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Sodium using this Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1 validated for use in ELISA, IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: It is recommended that the antibody be tried at 2-20 µg/mL on paraformaldehyde fixed tissue. ELISA: 0.5-1.0 µg/mL of a previous lot was used with 1 µg/mL control peptide per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 22 amino acid peptide sequence near the C-terminus of rat NKCC1. (Moore-Hoon et al. 1998; Delpire et al. 1994; Payne et al. 1995; Isering et al. 1997; Mount et al. 1998). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat Sodium, Potassium, Chloride Cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC. |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Expected to cross-react with human and mouse. The immunogen sequence is 95% conserved in mouse, 86% in human, and 82% in bovine. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | By moving chloride into epithelial cells, the Na-K-Cl cotransporter SLC12A2 aids transcellular movement of chloride across both secretory and absorptive epithelia (Payne et al., 1995 [PubMed 7629105]). See also SLC12A1 (MIM 600839) and SLC12A3 (MIM 600968).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. SIZE: 1,205 amino acids; 130,950 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in many tissues. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the SLC12A transporter family. |
| Molecular Weight | 170 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Rat Spinal Cord lysates. Western Blot Analysis: 1:500 dilution detected NA+K+CL- COTRANS 1 on 10 µg of Rat Spinal Cord lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |