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Millipore Anti-Acetyl-Mvh (Lys405) Antibody Is A Rabbit Polyclonal Antibody For Detection Of Acetyl-Mvh (Lys405) Also Known As Probable Atp-Dependent Rna Helicase Ddx4, Dead Box Protein - Mill (Additional S&H or Hazmat Fees May Apply)
MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.
Product Information
Format
Affinity Purified
Control
Non-acetylated and acetylated MVH (Lys405)
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.
Key Applications
Dot Blot
Western Blotting
Immunocytochemistry
Peptide Inhibition Assay
Immunoprecipitation
Application Notes
Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.
Epitope
Acetylated Lys405
Host
Rabbit
Specificity
This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.
Species Reactivity
Mouse
Human
Rat
Pig
Species Reactivity Note
Demonstrated to react with Mouse and Human. Predicted to react with Rat and Porcine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_034159
Gene Symbol
Ddx4
Vasa
Modifications
Acetylation
Purification Method
Affinity Purfied
UniProt Number
Q61496
UniProt Summary
FUNCTION: May play a role in germ cell development. May play a role in sperm motility.
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL.
Molecular Weight
76 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).
Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
