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Millipore Anti-Zscan4 Antibody Is A Highly Specific Rabbit Polyclonal Antibody, That Targets Zinc Finger Protein & Has Been Tested In Western Blotting, Immunofluorescence & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
Zinc finger and SCAN domain containing protein 4, also called Zscan4, is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency. Zscan4 binds and regulates telomere elongation in order to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells.
Product Information
Format
Affinity Purified
Control
Embryonic Stem Cell lysates
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Zscan4 Antibody is a highly specific rabbit polyclonal antibody, that targets Zinc Finger Protein & has been tested in western blotting, Immunofluorescence & IP.
Key Applications
Western Blotting
Immunofluorescence
Immunoprecipitation
Application Notes
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Zscan4 in mouse iPS and R1 Embyonic Stem cells. Data courtesy of the Melanie Welham lab at The University of Bath, UK Immunoprecipitation Analysis: A 1:4,000 dilution from a representative lot detected Zscan4 in cytoplasmic and nuclear extracts in IP experiments conducted by the Melanie Welham lab at The University of Bath, UK. (Data not shown) Western Blotting Analysis: A representative lot of this antibody was used to detect Zscan4 in Wild-type (WT) and Gsk-3 double knock-out (DKO) mouse embryonic stem cells (ESCs) (Storm, M.P., et al. (2014). PLoS One. 9(3):e89821).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to mouse Zscan4.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001013787
Gene Symbol
Zscan4c
Gm397
Purification Method
Affinity Purfied
UniProt Number
Q80VJ6
Molecular Weight
~65 kDa observed. The calculated molecular weight of this protein is 57 kDa. An uncharacterized band may be observed at ~50 kDa in some cell lysates.ZSCAN4 bands may be observed at both ~72 kDa and ~40 kDa when preparing cell lysates with SDS buffer. Additionally, a ZSCAN4 doublet may be observed at ~72 kDa when using cytosolic or nuclear extracts.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse Embryonic Stem cell lysates.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Zscan4 in 10 µg of Embryonic Stem cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
