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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Eu | IHC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5010 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Glutathione Antibody, detects both GSH & GSSG |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | IgG fraction in sterile 0.1M phosphate buffer. No preservative |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Glutathione Antibody, detects both GSH & GSSG is validated for use in IH for the detection of Glutathione. |
| Key Applications |
|
| Application Notes | Immunohistochemistry using silver-intensified immunogold or enhanced fluorescence methods. Treatment with autofluorescent reducers is typically necessary when using glutaraldehyde fixatives. Samples should be fixed with 0.2% - 2.5% glutaraldehyde for optimum detection. See notes technical notes. This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5010 is 0.02%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used (only in blocking buffer, antibody dilution buffer should be in simple TBS or PBS only). Western blot (Mawatari and Murakami, 2004). Optimal working dilutions must be determined by the end user. Note: Glutathione is not osmium tolerant and therefore should not be used for post-embedding electron micoscope immunohistochemistry. All procedures may be carried out at room temperature. Though the exact dilutions for all applications can not be predicated, it is unlikely that deviations from the calibrated concentration will be needed. The 100X dilution is calibrated to optimize the detection of antigen levels over a 2 log unit range. Greater or lesser dilutions may be employed if unusually high (>20 mM) or low (<0.2 mM) intracellular hapten levels are anticipated or novel specimen conditions exist, such as low glutaraldehyde levels or unusual embedding protocols. Greater dilutions will likely result in decreased maximum signal strength whereas more concentrated solutions will compress the dynamic range of detection. TECHNICAL NOTES The AB5010 is optimized for use with immunogold or fluorescence detection. Visualization with enzyme-linked methods can be used but they typically compress the dynamic range of detection. Use of high gain methods such as tyramides will require the user to perform independent calibrations. Use with frozen sections is possible but will not yield optimal images as IgGs will penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effect occurs. Use in whole mount or vibratome sections is not recommended for similar reasons. |
| Biological Information | |
|---|---|
| Immunogen | Glutathione-glutaraldehyde |
| Epitope | detects both GSH & GSSG |
| Host | Rabbit |
| Specificity | Glutathione. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, g-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Does not discriminate between free GSH and GSSG. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not refreeze this stock. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |
| Material Package | sufficient for 2000 assays |