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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5204 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I is validated for use in IH, WB for the detection of Sodium Channel. |
| Key Applications |
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| Application Notes | All procedures that are going to receive a full-length protein should be performed at 4C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mm), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each). Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat brain fixed frozen sections and mouse heart tissue. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified peptide from 465-481 of alpha subunit of rat type I voltage-gated sodium channel (VGSC) (Accession P04774). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested SO far. SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested. |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials, chiefly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. Functional studies have indicated that the transmembrane alpha subunit of the brain sodium channels is sufficient for expression of functional sodium channels (Goldin et Al., 1986 [PubMed 2429308]; Isom, 2002 [PubMed 11779698]).[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P35498 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. SIZE: 2009 amino acids; 228972 Da SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. DOMAIN: SwissProt: P35498 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. DISEASE: SwissProt: P35498 # Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a severe form of generalized epilepsy with febrile seizures. It is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. & Defects in SCN1A are the cause of familial hemiplegic migraine 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia and phonophobia. Age at onset ranges from 6 to 15 years. Some patients may manifest seizures during infancy. & Defects in SCN1A are the cause of familial febrile convulsions 3 (FEB3) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Inheritance pattern is autosomal dominant. SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |