The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Quality Level
MQ100
Applications
Application
This Anti-Syntaxin 4 Antibody is validated for use in WB for the detection of Syntaxin 4.
Key Applications
Western Blotting
Application Notes
Western blotting: 0.5-1.0 μg/mL (1:200-1:500) Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Highly purified corresponding to residues 2-23 of rat or mouse Syntaxin 4 (Accession Q08850).
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Recognizes Syntaxin 4.
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_004604.3
Gene Symbol
STX4
p35-2
STX4A
Syntaxin-4
UniProt Number
Q12846
UniProt Summary
FUNCTION: SwissProt: Q12846 # Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones. SIZE: 297 amino acids; 34180 Da SUBUNIT: Binds STXBP3 and STXBP6. Binding to STXBP3 excludes binding with SNAP25. Interacts with SNAP23 and SNAP25BP. Found in a complex with VAMP8 and SNAP23 in pancreas (By similarity). Interacts with VAMP2 and STXBP4 (By similarity). Detected in a complex with SNAP23 and STXBP4. Interaction with STXBP4 excludes interaction with VAMP2 (By similarity). Interacts with STX4A (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane protein (Potential). SIMILARITY: SwissProt: Q12846 ## Belongs to the syntaxin family. & Contains 1 t-SNARE coiled-coil homology domain.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
