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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5360-200UL |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Potassium Channel Kv4.2 Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Potassium Channel Kv4.2 Antibody is validated for use in WB for the detection of Potassium Channel Kv4.2. |
| Key Applications |
|
| Application Notes | Western blotting: 1.0-1.5 μg/mL (1:200-1:300) Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Highly purified peptide corresponding to residues 454-469 of rat Kv4.2 (Accession Q63881). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Kv4.2. The epitope specific for Kv4.2 is not present in any other known proteins. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9NZV8 # Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. SIZE: 630 amino acids; 70537 Da SUBUNIT: Homotetramer or heterotetramer with KCND1 and/or KCND3. Interacts with DPP6, DLG4 and FREQ (By similarity). Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Interacts with FLNA, FLNC and DPP10. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression. TISSUE SPECIFICITY: Highly expressed throughout the brain. Expression is very low or absent in other tissues.DOMAIN:SwissProt: Q9NZV8 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. PTM: Phosphorylated on serine and threonine residues (By similarity). SIMILARITY: Belongs to the potassium channel family. D (Shal) subfamily. |
| Molecular Weight | 70 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |