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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ICC, IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5382 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Nitric Oxide Synthase II Antibody |
| Alternate Names |
|
| Background Information | Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: Nterminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and Cterminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaMbinding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum in buffer containing liquid 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver). Immunocytochemistry: 1:50,000 dilution of a previous lot was used on cultured cells. Western blot: 1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | C-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse and rat. Reactivity to human iNOS is approximately 1%. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity). SIZE: 1153 amino acids; 131117 Da SUBUNIT: Homodimer. Binds SLC9A3R1. TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. |
| Molecular Weight | 131 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Mouse liver lysates. Western Blot Analysis: 1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |