Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | IH(P), WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5411 |
| Replaces | AB5386 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Nitrotyrosine Antibody |
| Background Information | The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody validated for use in IH(P) & WB. |
| Key Applications |
|
| Application Notes | Western Blot: 1:100-1:1,000 dilution of a previous lot was used sucessfully in western blot when tested against nitrated BSA and nitrated ovalbumin. Immunohistochemistry: 1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the AB5411 was for 10 minutes and the reaction was developed with the Chemicon IHCSelect, HRP-DAB kit (Catalog number DAB150). Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Nitrated KLH. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes nitrated tyrosine residues. |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Reactivity with other species has not been determined. |
| Antibody Type | Polyclonal Antibody |
| Purification Method | Protein A Purfied |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry(paraffin) Analysis: Nitrotyrosine (AB5411) staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen in here in hippocampus. Optimal Staining of Nitrotyrosine Monoclonal: Mouse Brain Treated With Zymosan. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |