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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Dr | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5436P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Brain and Muscle ARNT-Like 1 Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Brain & Muscle ARNT-Like 1 Antibody is a Rabbit Polyclonal Antibody for detection of Brain & Muscle ARNT-Like 1 also known as BMAL1, MOP3/CYCLE & has been validated in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 22 amino acid peptide of drosophila BMAL1 (Darlington et al. 1998; Rutila et al. 1998; Bae et al. 1998). Gene accession # 3219728. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes drosophila BMAL1. The immunogen shows no significant sequence homology with mammalian Clock or Per proteins. Significant homology is seen with mammalian USFs (Upstream Stimulatory Factors 1 and 2) and human and mouse BMAL1 and ARNTs. SPECIES REACTIVITIES: The immunogen sequence has no homology with mammalian Clock or Per proteins. Reactivity with other species has not been confirmed. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O00327 # BMAL1-CLOCK heterodimers activate E-box element (3- CACGTG-5) transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins (By similarity). SIZE: 626 amino acids; 68762 Da SUBUNIT: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with CLOCK is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and BMAL1. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-BMAL1 heterodimer with PER or CRY inhibits transcription activation. Interacts with HSP90; with AHR in vitro, but not in vivo. SUBCELLULAR LOCATION: Nucleus (By similarity). TISSUE SPECIFICITY: Highly expressed in the adult brain, skeletal muscle and heart. PTM: Phosphorylated upon dimerization with CLOCK (By similarity). & Sumoylated on Lys-259 upon dimerization with CLOCK (By similarity). SIMILARITY: SwissProt: O00327 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains. MISCELLANEOUS: CLOCK-BMAL1 double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |