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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB, ICC, IHC | Sh | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5447 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tropomyosin Antibody, 5NM1 and 5NM2 |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin in PBS (liquid). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Tropomyosin using this Anti-Tropomyosin Antibody, 5NM1 & 5NM2 validated for use in WB, IC, IH. |
| Key Applications |
|
| Application Notes | Western Blot: 1:100-1:500. Antibody reacts with a band at 29-30kDa. Immunocytochemistry: 1:100-1:500 Immunohistochemistry: 1:100-1:500 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the 9d exon from the gamma gene of tropomyosin. |
| Epitope | 5NM1 and 5NM2 |
| Host | Sheep |
| Specificity | Recognizes Tropomyosin 5NM1 and 5NM2. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P06753 # Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. SIZE: 284 amino acids; 32819 Da SUBUNIT: Heterodimer of an alpha and a beta chain. Binds to TMOD1. DOMAIN: SwissProt: P06753 The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven- residues periodicity. DISEASE: SwissProt: P06753 # Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1) [MIM:609284]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. NEM1 inheritance is autosomal dominant. & A chromosomal aberration involving TPM3 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. A rearrangement with NTRK1 generates the TRK fusion transcript by fusing the amino end of isoform 2 of TPM3 to the 3-end of NTRK1. SIMILARITY: Belongs to the tropomyosin family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |