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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Mk, R, Fe | ICC, IHC, IP, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5454 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-TUC-4 Protein Antibody |
| Alternate Names |
|
| Background Information | TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (Quinn, 1999). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (Minturn, 1995a). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (Minturn, 1995b). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (Quinn, 1999). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (Minturn, 1995a). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (Minturn, 1995b) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR; Gaetano, 1997). |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit Serum. Liquid containing 0.2% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TUC-4 Protein Antibody detects level of TUC-4 Protein & has been published & validated for use in IC, IH, IP & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:1,000-1:5,000 using PFA (DAB detection). Immunocytochemistry: 1:1,000-1:5,000 (DAB detection). Western blot: 1:2,500-1:25,000 using alkaline phosphatase. Immunoprecipitation Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK) |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Specifically recognizes the TUC-4 (Ulip-1/CRMP-4/DRP-3) splice variants, TUC-4a (64 kDa) and the N-terminally extended TUC-4b (75 kDa) (Quinn, 2003). AB5454 has been reported to label early postmitotic neurons in prenatal rat brain. Labeling is observed primarily within the cytoplasm along the most distal regions of neuronal growth cones including lamellipodia and filopodia. The antibody is not reactive with the proteins TUC-1 (Ulip-3/CRMP-1), TUC-2 (Ulip-2/CRMP-2) or TUC-3 (Ulip-4/CRMP-3). It does not label neural progenitors or radial glia. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q14195 # May regulate vesicle function in the growth cone (By similarity). SIZE: 570 amino acids; 61963 Da SUBUNIT: Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, growth cone (By similarity). Note=Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone (By similarity). TISSUE SPECIFICITY: Mainly in heart and skeletal muscle. Also expressed strongly in fetal brain and spinal cord. SIMILARITY: SwissProt: Q14195 ## Belongs to the dehydropyrimidinase family. |
| Molecular Weight | 64 & 74 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |