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Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.
Presentation
Liquid rabbit serum containing no preservative.
Quality Level
MQ100
Applications
Application
Anti-AANAT Antibody is an antibody against AANAT for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.
Host
Rabbit
Specificity
Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.
Species Reactivity
Bovine
Human
Rat
Sheep
Fish
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_001088.1
Entrez Gene Summary
Arylalkylamine N-acetyltransferase belongs to the superfamily of acetyltransferases. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle.
Gene Symbol
AANAT
SNAT
AA-NAT
EC 2.3.1.87
UniProt Number
Q16613
UniProt Summary
FUNCTION: SwissProt: Q16613 # Catalyzes the N-acetylation of serotonin into N- acetylserotonin. SIZE: 207 amino acids; 23344 Da SUBUNIT: Monomer (By similarity). TISSUE SPECIFICITY: Highly expressed in pineal gland and retina. SIMILARITY: SwissProt: Q16613 ## Belongs to the acetyltransferase family. AANAT subfamily. & Contains 1 N-acetyltransferase domain.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
