Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, Zebrafish, M | ICC, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5475 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Prox 1 Antibody |
| Background Information | Prox1, the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit serum in 50 % glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Prox 1 using this Anti-Prox 1 Antibody validated for use in IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum. Immunocytochemistry: A previous lot of this antibody was used on Immunocytochemistry |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from the C-terminus of mouse Prox1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Prox1. The antibody is useful for identifying Prox1 expressing cells during the development of many areas of the nervous system including the dorsal spinal cord, ganglionic eminences, thalamus and hippocampus. |
| Species Reactivity |
|
| Species Reactivity Note | Rat, mouse and zebrafish. The antibody is also expected to react with human. Other species have not been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q92786 # May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons (By similarity). SIZE: 737 amino acids; 83203 Da SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. SIMILARITY: SwissProt: Q92786 ## Belongs to the Prospero homeobox family. & Contains 1 Prospero-type homeobox DNA-binding domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |