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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, Pm | ICC, IHC | Gp | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5509 |
| Replaces | AB1774 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Opioid Receptor µ Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | PBS containing 50% glycerol; Concentration 1.0 mg/ml |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Opioid Receptor μ Antibody-AB5509 is an antibody against Opioid Receptor for use in IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:50-500 See Protocol. Immunocytochemistry: 1:50-500 See Protocol. Optimal working dilutions must be determined by the end user. IHC Protocol: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5509. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. ICC Protocol: Mu opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum free media. Media was removed and cells were gently washed 3 times with serum free media. Following fixation, cells were processed for indirect immunofluorescence as described above. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide (Catalog number AG375) from the C-terminus of rat mu Opioid receptor corresponding to residues 384-398 of the rat mu opioid receptor. |
| Host | Guinea Pig |
| Specificity | Mu Opioid receptor. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P35372 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin. SIZE: 400 amino acids; 44779 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P35372 ## Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |