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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ch | IHC, WB, ChIP, ChIP-seq, ICC, IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5535 |
| Replaces | AB5809 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Sox9 Antibody |
| Background Information | Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Sox9 in murine embryonic bone and adult cartilage tissue sections. Western Blotting Analysis: An 1:500 dilution from a representative lot detected Sox9 in human PC3 prostate cancer cells and HepG2 hepatocytes. Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at target chromatin sites by ChIP using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at the Bmi promoter in Z/sox9tg but not in wild-type control mouse embryonic fibroblasts/MEFs (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315). ChIP-sequencing (ChIP-seq) Analysis: A representative lot detected Sox9-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243). Immunofluorescence Analysis: A representative lot detected the accumulation of Sox9-positive oval cells by fluorescent immunohistochemistry staining of paraffin-embedded liver sections from transgenic mice treated with diethylnitrosamine to induce conditional liver HNF4a knockout (Saha, S.K., et al. (2014). Nature. 513(7516):110-114). Immunofluorescence Analysis: Representative lots detected Sox9 immunoreactivity in paraffin-embedded mouse embryo sections by fluorescent immunohistochemistry (Carrasco, M., et al. (2012). J. Clin. Invest. 122(10):3504-3515; Sylva, M., et al. (2011). PLoS One. 6(8):e22616). Immunofluorescence Analysis: Representative lots immunostained Müller glial cells in frozen mouse and chicken retina sections by fluorescent immunohistochemistry staining of (Muranishi, Y., and Furukawa, T. (2012). J. Biomed. Biotechnol. 2012:973140; Fischer, A.J., et al. (2011). Neuroscience. 178:250-260). Immunocytochemistry Analysis: A representative lot detected the stem cell marker Sox9 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441). Immunohistochemistry Analysis: A representative lot immunostained the supporting cells (Sertoli) of the seminiferous tubules by immunohistochemistry staining of paraffin-embedded mouse testis sections (OShaughnessy, P.J., et al. (2012). PLoS One. 7(4):e35136). Immunohistochemistry Analysis: A representative lot detected Sox9 immunoreactivity in various formalin-fixed, paraffin-embedded human tumor tissue sections (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315). Western Blotting Analysis: A representative lot detected the stem cell marker Sox9 in E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441). Western Blotting Analysis: A representative lot detected upregulated Sox9 expression level in human colorectal cancer cell lines, HCT116, DLD1, and SW620 (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Sox9. |
| Epitope | C-terminal |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Sox9. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat, Chicken. Predicted to react with Bovine, Equine, Feline, and Ovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P48436 # Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. SIZE: 509 amino acids; 56137 Da SUBCELLULAR LOCATION: Nucleus (Potential). DISEASE: SwissProt: P48436 # Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. SIMILARITY: SwissProt: P48436 ## Contains 1 HMG box DNA-binding domain |
| Molecular Weight | 56-65 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: An 1:2000 dilution of this antibody detected Sox9 in HepG2 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |