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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, Po, R, Fe | ICC, IHC, IH(P), WB | Ch | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5539 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Neurofilament H Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified chicken IgY in PBS containing 5mM sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa. Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde. Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified bovine NF-H. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Chicken |
| Specificity | Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Ammonium Sulfate Precipitation |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. SIZE: 1026 amino acids; 112480 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family. |
| Molecular Weight | 200 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |