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Positive = cerebral cortex. Negative = liver or kidney.
Presentation
Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.
Quality Level
MQ100
Applications
Application
Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.
Key Applications
Immunocytochemistry
Immunohistochemistry
Western Blotting
Application Notes
Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.
Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.
Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Purified bovine brain MAP2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Chicken
Specificity
Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_001039538.1
NM_002374.3
NM_031845.2
NM_031847.2
Entrez Gene Summary
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
Gene Symbol
MAP2
MAP2B
MAP-2
MAP2C
DKFZp686I2148
MAP2A
Purification Method
Ammonium Sulfate Precipitation
UniProt Number
P11137
UniProt Summary
FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Molecular Weight
Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
