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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5559 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-GABA A Receptor γ2 Antibody, cytoplasmic loop |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol. Approximate concentration is 0.2 mg/mL. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GABA A Receptor γ2 Antibody, cytoplasmic loop detects level of GABA A Receptor γ2 & has been published & validated for use in IP & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 Immunopreciptation: The antibody will immunoprecipitate 49% of all GABAA receptors in dodecylsulfate extracts of rat forebrains (150 μL) using 15 μg of antibody. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | MBP fusion protein from the cytosolic loop of rat GABAA receptor gamma2 subunit. |
| Epitope | cytoplasmic loop |
| Host | Rabbit |
| Specificity | GABAA receptor gamma2 subunit. Reacts with the ~44-47kDa gamma2 subunit of the GABAA receptor in rat brain membrane fractions. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the brain, mediates neuronal inhibition by binding to GABA receptors. The type A GABA receptors are pentameric chloride channels assembled from among many genetic variants of GABA(A) subunits. This gene encodes the gamma 2 subunit of GABA(A) receptor. Mutations in this gene have been associated with epilepsy and febrile seizures. Alternative splicing of this gene results in transcript variants encoding different isoforms. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P18507 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. SIZE: 467 amino acids; 54162 Da SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Interacts with GABARAP. SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. PTM: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability (By similarity). DISEASE: SwissProt: P18507 # Defects in GABRG2 are the cause of childhood absence epilepsy type 2 (ECA2) [MIM:607681]. ECA2 is a subtype of idiopathic generalized epilepsy (IGE) characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Some individuals manifest ECA2 occurring in combination with febrile seizures [MIM:121210]. A febrile seizure is defined as a convulsion event in infancy or childhood, usually occurring between 3 months and 5 years of age, associated with fever but without any evidence of intracranial infection or defined pathologic or traumatic cause. & Defects in GABRG2 are the cause of generalized epilepsy with febrile seizures plus type 3 (GEFS+3) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in GABRG2 are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]. SMEI is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. SIMILARITY: SwissProt: P18507 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family. MISCELLANEOUS: This subunit carries the benzodiazepine binding site. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |