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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5932 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Potassium Channel KvLQT1 Antibody, CT |
| Alternate Names |
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| Background Information | KvLQT1(Kv7.1) is a voltage-gated potassium channel protein coded for by the gene KCNQ1. This protein is present in the cell membranes of cardiac muscle tissue and in inner ear neurons among other tissues. KvLQT1 can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3 and in heart cells is required for the repolarization phase of the cardiac action potential. Mutations in the gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation, lead to a defective KvLQT1 protein and several forms of inherited arrhythmias known as Long QT syndrome, Short QT syndrome, and Familial Atrial Fibrillation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Potassium Channel KvLQT1 Antibody, C-terminus detects level of Potassium Channel KvLQT1 & has been published & validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Potassium Channel KvLQT1 in human myocardium tissue. /Western Blotting Analysis: 2 μg/mL of this antibody detected KvLQT1 in 10 μg of human heart tissue lysate. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide from the C-terminus of human KvLQT1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes Potassium Channel KvLQT1 protein. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with rat and mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein for a voltage-gated potassium channel required for the repolarization phase of the cardiac action potential. The gene product can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation. The gene is located in a region of chromosome 11 that contains a large number of contiguous genes that are abnormally imprinted in cancer and the Beckwith-Wiedemann syndrome. Two alternative transcripts encoding distinct isoforms have been described. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Probably important in cardiac repolarization. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current in CHO Cells in which cloned KCNQ1/KCNE1 channels were coexpressed with M1 muscarinic receptors. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea. SIZE: 676 amino acids; 74699 Da SUBUNIT: Heteromultimer with KCNE1 (MinK) or KCNE3 (MiRP2). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. DISEASE: Defects in KCNQ1 are the cause of long QT syndrome type 1 (LQT1) [MIM:192500]; also known as Romano-Ward syndrome (RWS). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. LQT1 inheritance is an autosomal dominant. & Defects in KCNQ1 are a cause of Jervell and Lange-Nielsen syndrome (JLNS) [MIM:220400]. JLNS is an autosomal recessive cardio-auditory syndrome characterized by a prolonged QT interval in the electrocardiogram and congenital deafness. & Defects in KCNQ1 are the cause of atrial fibrillation type 1 (ATFB1) [MIM:607554]. ATFB is characterized by rapid and irregular activation of the atrium. ATFB causes thromboembolism, tachycardia-mediated cardiomyopathy, heart failure and ventricular arrhythmia. & Defects in KCNQ1 are the cause of short QT syndrome type 2 (SQT2) [MIM:609621]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SIMILARITY: Belongs to the potassium channel family. KQT subfamily. MISCELLANEOUS: Mutagenesis experiments were carried out by expressing in Xenopus oocytes or COS-7 cells KCNQ1 mutants either individually (homomultimers) or in combination with both wild-type KCNQ1 (mut/wt homomultimers) and minK (heteromultimers). |
| Molecular Weight | ~ 72 kDa Observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human heart tissue lysate. Western Blot Analysis: 2 µg/mL of this antibody detected KvLQT1 in 10 µg of human heart tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |