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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5972 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Dishevelled-2 Antibody |
| Alternate Names |
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| Background Information | DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present. Immunohistochemistry: 1:1,000 using the avidin-biotin system. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody). Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein. |
| Host | Rabbit |
| Specificity | Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14641 # May play a role in the signal transduction pathway mediated by multiple Wnt genes. SIZE: 736 amino acids; 78948 Da SUBUNIT: Interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2 (By similarity). Interacts with DIXDC1 and Rac. SUBCELLULAR LOCATION: Cytoplasm. PTM: Phosphorylated. SIMILARITY: SwissProt: O14641 ## Belongs to the DSH family. & Contains 1 DEP domain. & Contains 1 DIX domain. & Contains 1 PDZ (DHR) domain. |
| Stem Cell Type |
|
| Molecular Weight | 80 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |