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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6002 |
| Replaces | 04-1122 |
| Description | Anti-MMP-1 Antibody, hinge region |
| Alternate Names |
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| Background Information | All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). MMP1, also known as interstitial collagenase, is the only enzyme able to initiate the breakdown of the interstitial collagens, types I, II, and III. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MMP-1 Antibody, hinge region is an antibody against MMP-1 for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide corresponding to the hinge region of human MMP-1. |
| Epitope | Hinge region |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes an internal domain of human MMP-1 containing the hinge region. It does not cross react with MMP-2, MMP-9, or MMP-13. |
| Species Reactivity |
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| Species Reactivity Note | Human. Additional sequence homology is noted with mouse (81%) and rat (75%). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| Gene Symbol |
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| Purification Method | Peptide affinity purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tats mediated neurotoxicity. COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit. SIZE: 469 amino acids; 54007 Da SUBUNIT: Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Secreted. DOMAIN: There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases). & The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. |
| Molecular Weight | 54 kDa. In Western Blot, bands around 53 kDa and 51 kDa (proenzyme) are observed in reduced proteins. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated on a representative lot by Western blot on A431 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected MMP-1 on 10 µg of A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |