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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Eq, Ca, B | WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6020 |
| Description | Anti-MAP4 Antibody |
| Alternate Names |
|
| Background Information | Microtubule-associated protein 4 (MAP4) is a ubiquitous non-neuronal microtubule-associated protein. This protein binds, polymerizes, and stabilizes microtubules and is thought to regulate microtubule dynamics during the cell cycle. p34cdc2 kinase phosphorylation of MAP4 appears to regulate the control of microtubule reorganization at mitosis (Ookata, 1997). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MAP4 Antibody is validated for use in WB, IC for the detection of MAP4. |
| Key Applications |
|
| Application Notes | Western blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected MAP4 in 10 µg of HeLa cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MAP4 in HeLa, NIH/3T3, and A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated peptide corresponding to the C-terminus of human MAP4. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Antibody recognizes the C-terminus of MAP4. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human, mouse, and rat. Predicted to react with equine, canine, and bovine based upon 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Non-neuronal microtubule-associated protein. Promotes microtubule assembly. SUBUNIT STRUCTURE: Interacts with SEPT2; this interaction impedes tubulin-binding. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338. SEQUENCE SIMILARITIES: Contains 4 Tau/MAP repeats. |
| Molecular Weight | ~ 220 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western blot in HeLa cell lysate. Western blot Analysis: 0.05 µg/mL of this antibody detected MAP4 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |