Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rhesus Macaque, Pm, B | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6046 |
| Description | Anti-Cytoplasmic FMR1-interacting protein 1 Antibody |
| Alternate Names |
|
| Background Information | Cytoplasmic FMR1-interacting protein 1 (CYFIP1) is a member of the CYFIP family. It is a component of the CYFIP1-EIF4E-FMR1 complex, a regulatory complex for translational repression that binds to the mRNA cap. Within the complex, CYFIP1 serves as an adapter between the other two components and upregulates the translation repression activity of FMR1. It regulates epithelial morphogenesis, binds F-actin, and is a component in the development and formation of axon outgrowth, lamellipodia, protrusions, and membrane ruffles. Through its interaction with RAC1, CYFIP1 regulates the activity of WASP family members. CYFIP1 is associated with Prader-Willi/Angelman syndromes and may also have a role in autism. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cytoplasmic FMR1-interacting protein 1 using this Anti-Cytoplasmic FMR1-interacting protein 1 Antibody validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Western Blot (Snap i.d.®) Analysis: 5 µg/mL from a previous lot detected Cytoplasmic FMR1-interacting protein 1 in 10 µg of HeLa cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cytoplasmic FMR1-interacting protein 1 in NIH/3T3, HeLa, and A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an internal sequence (a.a.519-532) of human Cytoplasmic FMR1-interacting protein 1. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human and mouse. Predicted to react with primate, rhesus macaque, bovine, and rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA By similarity. Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers. SUBUNIT STRUCTURE: Interacts with the active GTP-bound form of RAC1. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with FMR1 but does not bind to related proteins FXR1 or FXR2. Interaction with EIF4E stimulates FMR1 binding. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2 By similarity. Interacts through its C-terminus with the C-terminus of DPYSL2/CRMP2 which is necessary for DPYSL2-induced axon outgrowth. SUBCELLULAR LOCATION: Cytoplasm › perinuclear region By similarity. Cell projection › lamellipodium By similarity. Cell projection › ruffle By similarity. Cell junction › synapse › synaptosome By similarity. Note: Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia By similarity. MISCELLANEOUS: Breakpoint hotspot for the Prader-Willi/Angelman syndromes and may be implicated in autism. Commonly altered in tumors. SEQUENCE SIMILARITIES: Belongs to the CYFIP family. SEQUENCE CAUTION: The sequence BAA07552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
| Molecular Weight | ~ 154 kDa observed. There are three noted isoforms with calculated MWs of 51 kDa, 94 kDa, and 145 kDa. Additional isoforms may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1 µg/mL of the antibody detected Cytoplasmic FMR1-interacting protein 1 on 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Packaging Information | |
|---|---|
| Material Size | 100 µg |