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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IF, RIA | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB763P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Collagen Type V Antibody |
| Overview | Collagen Type V extracted and purified from human placenta. Antibody shows less than 0.1% reactivity with human Collagen Types I, III, and IV. AB763P also shows 0.1% reactivity with human Fibronectin and mouse Laminin by radioimmunoassay. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Collagen Type V Antibody detects level of Collagen Type V & has been published & validated for use in ELISA, IF, RIA. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen human skin, liver and placenta tissues. Radio Immunoassay ELISA Optimal working dilution must be determined by end user. |
| Biological Information | |
|---|---|
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Human collagen type V: 100% Human collagen type I, II, & III: < 0.5% Human collagen type IV: 20% Human fibronectin, albumin, immunoglobulins: < 0.5%. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P20908 # Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. SIZE: 1838 amino acids; 183560 Da SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Sulfated on 40% of tyrosines. DISEASE: SwissProt: P20908 # Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS-I is a connective-tissue disorder characterized by loose-jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar cigarette-paper scars. Inheritance is autosomal dominant. & Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type II (EDS-II) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis. Inheritance is autosomal dominant. SIMILARITY: SwissProt: P20908 ## Belongs to the fibrillar collagen family. & Contains 1 laminin G-like domain. & Contains 1 TSP N-terminal (TSPN) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |