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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9076 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-IP3 Receptor 3 Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit serum. Liquid. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-IP3 Receptor 3 Antibody detects level of IP3 Receptor 3 & has been published & validated for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 3 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel. Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from the C-terminus of rat IP3 Receptor 3. |
| Host | Rabbit |
| Specificity | IP3 Receptor 3. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q14573 # Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. SIZE: 2671 amino acids; 304038 Da SUBUNIT: Homotetramer. Interacts with OPRS1, TRPC1, TRPC3 and TRPC4 (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in intestinal crypt and villus epithelial cells. DOMAIN: SwissProt: Q14573 The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand- binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. PTM: Phosphorylated on tyrosine residues (By similarity). SIMILARITY: Belongs to the InsP3 receptor family. & Contains 5 MIR domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |