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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, ICC, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9084 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Polycystin-L Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Polycystin-L Antibody is validated for use in WB, IC, IH for the detection of Polycystin-L. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 using ECL. The antibody reacts with the 90 kDa Polycystin1-L1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel. Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from the N-terminal of human Polycystin-L1. |
| Host | Rabbit |
| Specificity | Polycystin-L |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the polycystin protein family containing 11 transmembrane domains, a receptor for egg jelly (REJ) domain, and a polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain. The encoded protein may play a role in the male reproductive system. Alternative splice variants have been described but their biological nature has not been determined. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q8TDX9 # May have a role in the heart and in the male reproductive system. SIZE: 2849 amino acids; 315435 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential). TISSUE SPECIFICITY: Detected in testis and in fetal and adult heart. SIMILARITY: SwissProt: Q8TDX9 ## Belongs to the polycystin family. & Contains 1 GPS domain. & Contains 2 PKD domains. & Contains 1 PLAT domain. & Contains 1 REJ domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Packaging Information | |
|---|---|
| Material Size | 100 µL |