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POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
Presentation
Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.
Key Applications
Western Blotting
Application Notes
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Full length recombinant rat MARCKS.
Epitope
Complete sequence
Host
Rabbit
Specificity
Recognizes MARCKS.
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_002356.5
Entrez Gene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Gene Symbol
MARCKS
MACS
80K-L
FLJ14368
PRKCSL
phosphomyristin
MRACKS
PKCSL
FLJ90045
UniProt Number
P29966
UniProt Summary
FUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. SIZE: 332 amino acids; 31555 Da PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
