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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9332-50UL |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Transient Receptor Potential Vanilloid 4 Antibody, pain |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Transient Receptor Potential Vanilloid 4 Antibody, pain is validated for use in WB for the detection of Transient Receptor Potential Vanilloid 4. |
| Key Applications |
|
| Application Notes | Western blot: 1:200 using ECL on ND7/23 lysate. Immunohistochemistry on rat brain and mouse cerebellum frozen sections. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours. 3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 10 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600). |
| Biological Information | |
|---|---|
| Immunogen | Purified peptide from amino acids 853-871 of rat TRVP4 (Accession number Q9ERZ8). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes TRPV4 (Transient Receptor Potential Vanilloid 4). |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9HBA0 # Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SIZE: 871 amino acids; 98281 Da SUBUNIT: Homotetramer (Probable). Self-associates in a isoform- specific manner. Isoforms 1/A and 5/D but not isoform 2/B, 4/C and 6/E can oligomerize. Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). Note=Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. & Isoform 1: Cell membrane. & Isoform 5: Cell membrane. SIMILARITY: SwissProt: Q9HBA0 ## Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |