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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, IHC, WB | Ch | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9354 |
| Replaces | 04-1049 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Beta III Tubulin Antibody |
| Background Information | Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide. Four different antibodies were combined to make this product |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: 1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells. Immunohistochemistry: 1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues. Optimal working dilutions must be determined by the end user |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptides from human/rat Beta III Tubulin. |
| Epitope | (Similar to TuJ1 antigen) |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Chicken |
| Specificity | Recognizes Beta III Tubulin |
| Isotype | IgY |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 450 amino acids; 50433 Da SUBUNIT: Dimer of alpha and beta chains. DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family. |
| Molecular Weight | 55 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on mouse brain lysates. Western Blot: 1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |