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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB951-I |
| Replaces | AB951 |
| Description | Anti-Neuron Specific Enolase Antibody |
| Alternate Names |
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| Background Information | The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha-enolase), neuron-specific enolase (NSE; gamma,gamma-enolase, also known as 14-3-2 protein and NSP) and the short-lived heterodimer alpha, gamma-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa, which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors. NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Neuron Specific Enolase Antibody is a highly specific rabbit polyclonal antibody, that targets Neuron-Specific Enolase & has been tested in western blotting & IHC (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected in Neuron Specific Enolase in human thalamus tissue and glioma sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the near N-terminus of human Neuron Specific Enolase. |
| Epitope | Near N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes a region near the N-terminus of Neuron Specific Enolase. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human, Rat, and Mouse. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~47 kDa observed. Cat. No. AB951, Anti-Neuron Specific Enolase detects Alpha Enolase (ENO1) at ~39kDa. However, Neuron Specific Enolase has been observed at ~48 kDa (Braus, B., K., et al (2008). Vet Immunol Immunopathol 124(1-2):177-183.; Feo, S., et al. (2000). FEBS Lett. 473(1):47-52.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Neuron Specific Enolase in 10 µg of rat brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |