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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, R, Rb, Sh | WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9736 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-14-3-3 η Antibody |
| Background Information | Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, eta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimers. The 14-3-3 eta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimers and Parkinsons Disease. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Liquid. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-14-3-3 η Antibody detects level of 14-3-3 η & has been published & validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Western blot: 1:3,000 using ECL. Immunohistochemistry: 1:1,600-1:3,200 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from the N-terminal area of sheep 14-3-3 eta. |
| Host | Rabbit |
| Specificity | 14-3-3 eta. By Western blot the antibody recognizes the ~30 kDa protein. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5 UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. |
| Gene Symbol |
|
| Purification Method | Serum |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q04917 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. SIZE: 246 amino acids; 28219 Da SUBUNIT: Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. TISSUE SPECIFICITY: Expressed mainly in the brain and present in other tissues albeit at lower levels. SIMILARITY: SwissProt: Q04917 ## Belongs to the 14-3-3 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |