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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC102 |
| Description | Anti-CCN1/CYR61 Antibody |
| Alternate Names |
|
| Background Information | CCN1 (cysteine-rich 61 or CYR61), a ubiquitous angiogenic factor, is a member of the CNN family, which is also comprised of CTGF, WISP-1, WISP-2, and WISP-3. It is a multidomain protein, containing one each of CTCK, IGFBP, TSP type-1, and VWFC domains. CCN1 is associated with the extracellular matrix and functions as a ligand for integrin receptors. It is expressed in response to growth factor stimulation, and mediates various cellular processes, including cell adhesion, migration, proliferation, angiogenesis, extracellular-matrix remodeling, and placental development. Previous studies have indicated that it may also play a role in wound healing by influencing the expression of genes required for this process, such as TIMP1, PAI-1, VEGA-A, VEGA-C, MMP1, MMP3, and uPA. CCN1 has also been implicated in breast cancer. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CCN1/CYR61 using this Anti-CCN1/CYR61 Antibody validated for use in Western Blotting, IHC(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:200 dilution from a representative lot detected CCN1/CYR61 in human kidney tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the CTCK domain of human CCN1/CYR61. |
| Epitope | CTCK domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the CTCK domain of: CCN1/CYR61 |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Antigen Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at higher molecular weights due to glycosylation. A degradation fragment may be observed at ~15 kDa in some lysates. (Rother et Al., 2010) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected CCN1/CYR61 in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |