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Millipore Detect Phospho-Ulk1/Atg1 (Ser758) Using This Anti-Phospho-Ulk1/Atg1 (Ser758) Antibody Validated For Use In Western Blotting, Peptide Inhibition Assay. - Mill (Additional S&H or Hazmat Fees May Apply)
ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.
Product Information
Format
Affinity Purified
Control
A431 and serum starved A431 cell lysates.
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect phospho-ULK1/ATG1 (Ser758) using this Anti-phospho-ULK1/ATG1 (Ser758) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.
Key Applications
Western Blotting
Peptide Inhibition Assay
Application Notes
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-ULK1/ATG1 (Ser758) in serum-starved A431 cell lysates.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human ULK1/ATG1 phosphorylated at Ser758.
Epitope
Phosphorylated Ser758
Host
Rabbit
Specificity
This antibody recognizes the ULK1/ATG1 phosphorylated at Ser758.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Mouse, Rat, and Bovine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003556
Gene Symbol
ULK1
Purification Method
Affinity Purfied
UniProt Number
O75385
Molecular Weight
~120 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in A431 and serum starved A431 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected ULK1/ATG1 on 10 µg of A431 and serum starved A431 cell lysates..
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
