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Millipore Detect Tmem132a Using This Rabbit Polyclonal Antibody, Anti-Tmem132a Antibody Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Transmembrane protein 132A (TMEM132A), or HSPA5-binding protein 1 (HSPA5BP1), is thought to be involved in embryonic and postnatal development of the brain, and the increased cell death resistance induced by serum starvation in cultured cells. TMEM132A is known to regulate cAMP-induced GFAP gene expression through phosphorylation of STAT3.
Product Information
Format
Affinity Purified
Control
Neuro2A cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect TMEM132A using this rabbit polyclonal antibody, Anti-TMEM132A Antibody validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Neuro2A in human cerebellum and thalamus tissues.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TMEM132A.
Epitope
Cytoplasmic domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the cytoplasmic domain of TMEM132A.
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_060340
Gene Symbol
TMEM132A
HSPA5BP1
Purification Method
Affinity Purfied
UniProt Number
Q24JP5
Molecular Weight
~140 kDa observed. The calculated molecular weight of this protein is 110kDa. This protein has been observed at ~135 and ~170 kDa due to glycosylation, myristoylation, and phosphorylation (Kentaro, O. H., et al. (2003). J Biol Chem. 278(12): 10531-10537.). Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Bloting in Neuro2A cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected TMEM132A in 10 µg of Neuro2A cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
