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Millipore Detect Autophagy-Related Protein;Atg1 Using This Rabbit Polyclonal Antibody, Anti-Phospho Ulk1 Antibody (Thr574) Validated For Use In Western Blotting & Peptide Inhibition Assay. - Mill (Additional S&H or Hazmat Fees May Apply)
ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Autophagy-related protein;ATG1 using this rabbit polyclonal antibody, Anti-phospho ULK1 Antibody (Thr574) validated for use in western blotting & Peptide Inhibition Assay.
Key Applications
Western Blotting
Peptide Inhibition Assay
Application Notes
Western Blotting/Peptide Inhibition Analysis: 0.5 µg/mL from a representative detected phospho ULK1 in WB/PIA.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to mouse ULK1 phosphorylated at Thr574.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Predicted to react with Mouse, Rat, and Bovine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_033495
Gene Symbol
Ulk1
Purification Method
Affinity Purfied
UniProt Number
O70405
Molecular Weight
~120 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in untreated and Calyculin A with okadaic acid treated Jurkat cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho ULK1 (Thr574) in 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
