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Millipore Detect Immunity-Related Gtpase Family M Protein Using This Rabbit Polyclonal Antibody, Anti-Irgm Antibody Validated For Use In Western Blotting, Ihc (Paraffin) & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Immunity-related GTPase family M protein (IRGM), also known as Interferon-inducible protein 1 (IFI1) and LPS-stimulated RAW 264.7 macrophage protein 47 homolog (LRG-47), is a member of the interferon-inducible GTPase family. It induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. IRGM is expressed in colon, small bowel, and peripheral blood leukocytes. Mutations in IRGM have been assoicated with inflammatory bowel disease type 19 which is subdivided into Crohns disease and ulcerative colitis phenotypes.
Product Information
Format
Affinity Purified
Control
Human brain tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Immunity-related GTPase family M protein using this rabbit polyclonal antibody, Anti-IRGM Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunofluorescence
Application Notes
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected IRGM in human brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected IRGM in human brain cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the intermediate domain of human IRGM.
Epitope
intermediate domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the intermediate domain of IRGM.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Gene Symbol
IRGM
Purification Method
Antigen Affinity Purified
UniProt Number
A1A4Y4
Molecular Weight
~31 kDa observed. Uncharacterized bands may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected IRGM in 15 µg of human brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
