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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC477 |
| Description | Anti-YY1AP1 Antibody |
| Alternate Names |
|
| Background Information | YY1-associated protein 1 (YY1AP1), also known as Hepatocellular carcinoma susceptibility protein, Hepatocellular carcinoma-associated protein 2, is encoded by the gene name YY1AP1, HCCA2, and YY1AP. YY1AP1 has been demonstrated to enhance transcription activation by interacting with YY1. Recent studies have shown that YY1AP1 may play a role in cell cycle regulation. YY1AP1 interacts with YY1 and MAD2L2. YY1AP1 is expressed ubiquitously. YY1AP1 has been detected in small intestine, skeletal muscle, lung, pancreas, brain, stomach, spleen, colon and heart. At very low levels, YY1AP1 has been detected in healthy liver. YY1AP1 is highly expressed in most liver carcinomas. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-YY1AP1 antibody is validated for use in WB, IH for the detection of YY1AP1. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected YY1AP1 in 10 µg of C2C12, Hek293, HeLa, HepG2, human placenta, Huvec, Jurkat, L6, mouse brain, NIH-3T3, PC12, PC3, and Raw 264.7 lysate. Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected YY1AP1 in human cerebellum, mouse hindbrain, and mouse cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human YY1AP1 near the N-terminal. |
| Epitope | Near N-terminal |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~90 kDa observed. The endogenous YY1AP protein signal is found at 90 kDa, in agreement with the predicted protein size of 750 amino acids derived from open reading frame (Wang, C.Y., et al. (2004). J. Biol. Chem. 279:17750-17755). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected YY1AP1 in 10 µg of A431 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |