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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC519 |
| Description | Anti-FANCF Antibody |
| Alternate Names |
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| Background Information | Fanconi anemia group F protein (FANCF), also known as Protein FACF, and encoded by the gene symbol FANCF, is a DNA repair protein that may operate in a postreplication DNA repair or may be involved in a cell cycle checkpoint function. Recent studies have implicated that FACF may play an important role in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Mutations affecting the FANCF gene have been associated with Fanconi anemia complementation group F (FANCF), a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. This disorder is also associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FANCF is an antibody against FANCF for use in WB, ICC. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected FANCF in 293T cells were cotransfected with HA-tagged HES1 and FA coding vectors, HeLa cell extracts treated with L685,458 , 293T cells were cotransfected with FA gene expression plasmids as indicated with either empty, or BACE1 or HES1 coding vectors, 293T cells were cotransfected with FANCF expression plasmids along with increasing amounts of HES1 coding vector (Tremblay, C.S., et Al. (2008). Blood Journal. 112:P2062-2070). Immunocytochemistry Analysis: A representative lot detected FANCF in HeLa cells treated with the gamma-secretase inhibitor L685,458 (Tremblay, C.S., et Al. (2008). Blood Journal. 112:P2062-2070). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human FANCF. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected FANCF in 10 µg of MCF-7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |