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Hormonally up-regulated neu tumor-associated kinase
B19
Serine/threonine-protein kinase MAK-V
Background Information
Hormonally up-regulated neu tumor-associated kinase (HUNK) belongs to the protein kinase superfamily and is an SNF1-related serine/threonine kinase that has a unique COOH-terminal (C-terminal) part without homology to any known protein. This protein is highly expressed in the mammary gland, kidney, and central nervous system.
Product Information
Format
Affinity Purified
Control
SK-BR3 cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-HUNK Antibody is validated for use in WB, IH(P) for the detection of HUNK.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected HUNK in human brain and human cerebellum tissues.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human HUNK.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Other homologies: Mouse (87% sequence homology) and Rat (85% sequence homology).
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_055401
Gene Symbol
HUNK
MAKV
Purification Method
Affinity Purfied
UniProt Number
P57058
UniProt Summary
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Molecular Weight
~70 kDa observed. The calculated molecular weight is 80 kDa, however HUNK has been shown as a ~70 kDa band in western blots. (Gardner, H.P., et al. (2000). Development. 127:4493-4509.)
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in SK-BR3 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected HUNK on 10 µg of SK-BR3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
