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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC950 |
| Description | Anti-PSAT1 Antibody |
| Alternate Names |
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| Background Information | Phosphoserine aminotransferase (EC 2.6.1.52; UniProt Q9Y617; also known as Phosphohydroxythreonine aminotransferase, PSAT) is encoded by the PSAT1 (also known as NLS2, PSA, PSATD) gene (Gene ID 29968) in human. PSAT1 is a class-V pyridoxal-phosphate-dependent aminotransferase family member that catalyzes the reversible conversion of 3-phosphohydroxypyruvate and to 1-phosphoserine, as well as the conversion of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to 4-phosphonooxy-L-threonine, using, in both cases, L-glutamate as an amine donor, which is converted to 2-oxoglutarate. PSAT1 is involved in serine biosynthesis from glycolytic intermediates and PSAT1 upregulation is reported in non-small cell lung cancer (NSCLC) and colon cancer cells, while PSAT1 knockdoown is shown to suppress the proliferation of MDA-MB-468 and BT-20 breast cancer cells. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PSAT1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PSAT1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected PSAT1 in A431, HeLa, HepG2, and NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence within the region between the two pyridoxal phosphate-binding domains of human PSAT1. |
| Epitope | Region between the two pyridoxal phosphate-binding domains. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | The immunogen sequence is 100% conserved in murine PSAT1 (UniProt Q99K85) and present in both spliced isoforms of human PSAT1 (Uniprot Q9Y617). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rabbit based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~40 kDa observed. 40.42 kDa (human isoform 1; Alpha) and 35.19 kDa (human isoform 2; Beta), 40.47 kDa (mouse) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected PSAT1 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |