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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P), IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABD135 |
| Description | Anti-NANOS3 Antibody |
| Alternate Names |
|
| Background Information | Nanos homolog 3 (Nanos3), also known as NOS-3 or NOS3, belongs to the Nanos family. Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. Nanos3 has been shown to play an important role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Nanos3 has also been shown to be essential in the early stage embryo protecting the migrating primordial germ cells (PGCs) from apoptosis. Additionally, it is implied to maintain the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways and to affect cell proliferation by repressing translation of specific mRNAs. Nanos3 is expressed primarily in ovary, testis and brain. In the ovaries, Nanos3 is expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, Nonos3 is expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Nanos homolog 3 using this rabbit polyclonal antibody, Anti-NANOS3 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected NANOS3 in human brain tissue. Immunofluorescence Analysis: 20 μg/mL from a representative lot detected NANOS3 in human brain cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of human NANOS3. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the C-terminus of NANOS3. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~21 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~19 kDa and ~21 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected NANOS3 in 15 µg of human brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |