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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, PIA, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABD43 |
| Description | Anti-Apelin receptor Antibody |
| Alternate Names |
|
| Background Information | Apelin receptor (UniProt: P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID: 187) in human. Apelin receptor(AR), a monomeric transmembrane protein, is a class A GPCR that is widely distributed in human. It is also expressed in embryonic and induced pluripotent stem cells. It is activated by its cognate peptide ligand, apelin, and induces a wide variety of effects, including vasoconstriction and dilation, strengthening cardiac muscle contractility, angiogenesis, fluid homeostasis, and energy metabolism. It also plays role in heart morphogenesis and promotes angioblast migration towards embryonic midline. Apelin-AR signaling has cardioprotective effects and administration of apelin-13 to glucose-deprived cultured cardiomyocytes has inhibitory effects on apoptosis. Glutamic acid 20 and aspartic acid 23 at the extracellular N-terminus of AR are reported to be the first physiological sites that bind apelin. After exposure to apelin, the receptor is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. (Ref.: Hu, G., et al. (2021). Front. Physiol. 12; 632886; Wang, Z., et al. (2015). Sci. Rep. 5; 8170; Chapman, NA., et al. (2014). Biochem. Cell Biol. 92(6); 431-440). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Apelin receptor, Cat. No. ABD43, is a rabbit polyclonal antibody that detects Apelin receptor and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in Mouse cerebellum, Human cerebral cortex, and Mouse cerebral cortex tissue sections. Peptide Inhibition Assay: Target band detection in Mouse cerebellum tissue lysate was prevented by pre-blocking of a representative lot with the immunogen peptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 13 amino acids from the third cytoplasmic domain within the C-terminal half of human Apelin receptor. |
| Epitope | Cytoplasmic domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Apelin receptor. It targets an epitope within the third cytoplasmic domain from the C-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rhesus Macaque, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon. SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family. |
| Molecular Weight | ~56 kDa observed; 42.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Mouse cerebellum tissue lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apelin receptor in Mouse cerebellum tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |