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Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8) and Negative regulator of IL2 or NIL-2-A zinc finger protein is a member of the Zinc finger-Homeobox (ZFH)-family of repressors and acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.
Product Information
Format
Affinity Purified
Control
Mouse embryo e11 tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-ZEB1 detects levels of ZEB1 proteins & has been published & validated for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human ZEB1.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Mouse
Human
Bovine
Chicken
Frog
Species Reactivity Note
Demonstrated to react with Mouse. Predicted to react with Bovine, Chicken, and Frog based on 100% sequence homology. Predicted to react with Human based on immunogen design.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_110378
Gene Symbol
ZEB1
AREB6
TCF8
Purification Method
Affinity Purfied
UniProt Number
P37275
Molecular Weight
~200 kDa observed. The calculated molecular weight is 124 kDa, however ZEB1 has been shown at ~180-210 kDa in western blots (Dave, N., et al. (2011). J. Biol. Chem. 286(14):12024-12032).
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse embryo e11 tissue lysate.
Western Blot Analysis: 1.0 µg/mL of this antibody detected ZEB1 in 10 µg of mouse embryo e11 tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
