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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IP, IHC, DB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1304 |
| Description | Anti-monomethyl Histone H3 (Lys4) Antibody |
| Alternate Names |
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| Background Information | Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Histone H3 is acetylated on Lys14 by Gcn5, and histone H3 phosphorylation at Ser10 facilitates acetylation of Lys14 by Gcn5 in vitro. Moreover, Lys14 is involved in chromatin remodeling, and RSC4 (part of the RSC chromatin remodeling complex) contains essential tandem bromodomains that require H3 Lys14 acetylation for gene activation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-monomethyl Histone H3 (Lys4) is an antibody against monomethyl Histone H3 (Lys4) for use in WB, IP, IH, DOT Blot. |
| Key Applications |
|
| Application Notes | DOT Blot Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665). Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl Histone H3 (Lys4) in 500 µg of HeLa acid extract. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in human and rat testis tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of human monomethyl Histone H3 (Lys4). |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: 0.5 µg/mL of this antibody detected monomethyl Histone H3 (Lys4) in 10 µg of HeLa acid extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |