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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1329 |
| Description | Anti-Chromobox protein homolog 3 Antibody |
| Alternate Names |
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| Background Information | Chromobox protein homolog 3, also known as Chromobox protein homolog 3 (HECH), Heterochromatin protein 1 homolog gamma (HP1 gamma), or Modifier 2 protein, and encoded by the gene name CBX3, is implied to be involved in transcriptional silencing in heterochromatin-like complexes. Chromobox protein homolog 3 recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Chromobox protein homolog 3 may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Chromobox protein homolog 3 may also play a role in the formation of functional kinetochore through interaction with MIS12 complex proteins. Chromobox protein homolog 3 binds directly to CHAF1A and it has been shown to interact with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2, SP100, MIS12, DSN1, CBX5 via the chromoshadow domain, POGZ, CHAMP1, and ASXL1. Chromobox protein homolog 3 is phosphorylated by PIM1. Recent studies have implied that Chromobox protein homolog 3 is phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Chromobox protein homolog 3 antibody is validated for use in WB, IC;IP for the detection of Chromobox protein homolog 3. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Chromobox protein homolog 3 in 500 µg of HeLa nuclear extract. Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Chromobox protein homolog 3 in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to Chromo Domain 1 of human Chromobox protein homolog 3. |
| Epitope | Chromo Domain 1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Bovine, Monkey, Mouse, Rat, Horse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~22 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Chromobox protein homolog 3 in 10 µg of HeLa nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |