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Millipore This Anti-Fuse-Binding Protein 1 | Abe1330 Antibody Is Validated For Use In Wb, Ic For The Detection Of Fuse-Binding Protein 1 | Abe1330. - Mill (Additional S&H or Hazmat Fees May Apply)
FUSE-binding protein 1, also known as Far upstream element-binding protein 1 FBP, FUSE-binding protein 1, DNA helicase V, hDH V, and encoded by the gene name FUBP1, regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. FUSE may act both as activator and repressor of transcription. There are three known FUSE binding proteins (FBP1, FBP2, and FBP3). All three belong to an ancient family of single-stranded DNA binding proteins. These proteins are required for regulation of the c-myc proto-oncogene and have been shown to bind to an A/T-rich element (far-ustream element) located 1.7 kb upstream of the c-myc P2 promoter. Although their primary sequences are closely related, the FBP, FBP2, and FBP3 genes are located on different chromosomes in both mice and humans. FUSE binding proteins are believed to be multifunctional. Besides regulating the transcription of c-myc (and quite likely other targets), the FBP family has been shown to bind a variety of RNAs in vitro.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-FUSE-binding protein 1 | ABE1330 antibody is validated for use in WB, IC for the detection of FUSE-binding protein 1 | ABE1330.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected FUSE-binding protein 1 in HeLa and A431 cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human FUSE-binding protein 1 near the N-terminal.
Epitope
Near N-terminal
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Rat, Mouse, Primate, Feline, Horse based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003893
Gene Symbol
FUBP1
Purification Method
Affinity Purfied
UniProt Number
Q96AE4
Molecular Weight
~76 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FUSE-binding protein 1 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
