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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1372 |
| Description | Anti-OGG1 Antibody |
| Alternate Names |
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| Background Information | The protein named N-glycosylase/DNA lyase or alternatively, 8-oxoguanine (8-OxoG) DNA glycosylase or AP lyase, and encoded by the gene named OGG1/MMH/MUTM/OGH1, is a DNA repair enzyme that incises DNA at 8-oxoG residues and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. OGG1 also has beta-lyase activity that nicks DNA 3 to the lesion. OGG1 is localized to the nucleus and nucleoplasm; in UVA-irradiated cells it can be found in the nuclear speckles. There is also an isoform found in the mitochondrion. Interestingly, OGG1 appears to be play a critical role in repairing mtDNA damage; mtDNA damage is linked to mitochondrial dysfunction, increased oxidative stress, and insulin resistance in muscle cells, and studies using OGG1 knockouts or over expressing OGG1 demonstrate that OGG1 is important for proper mtDNA damage repair and that disruption of OGG1 can lead to increased mtROS (reactive oxygen species) production and subsequent regulation of downstream events leading to insulin resistance in skeletal muscle. OGG1, or rather defects in OGG1 function, are also associated with a number of carcinomas, particularly Renal Cell Carcinomas of various cell types. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-OGG1 Antibody is validated for use in Western Blotting for the detection of OGG1. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected recombinant protein OGG1 (Bjoras, M., et al. (1997). EMBO. 16(20):6314–6322). Western Blotting Analysis: A representative lot detected OGG1 in Melphalan-resistant cells (Sousa, M., et al. (2013). PLOS One. 8(2):e55493). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human OGG1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~38 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in recombinant protein OGG1. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant protein OGG1 in 1 µg of cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |